Fertility of undiluted ram epididymal spermatozoa stored for several days at 4°C
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چکیده
منابع مشابه
Leptin Receptor in Ram Epididymal Spermatozoa
This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then...
متن کاملeffect of three days storage of coated spermatozoa at cooling and adding seminal plasma on ram fertility
introduction artificial insemination (ai) has only been used as a supplement to natural mating. ai, when used in conjunction with accurate progeny testing schemes, can substantially increase the rate of genetic progress compared with that of natural service. moreover, the use of ai causes the limitation of the transmitted diseases. cervical insemination with frozen-thawed ram semen has not been...
متن کاملEffects of storage duration at 5 ºC and type of cryoprotectant on cooled and frozen-thawed ram epididymal spermatozoa
Freezing of ejaculated and epididymal spermatozoa is currently a subject of interest with the purpose of establishing an efficient gene banking model for valuable animals or endangered species. Therefore, the present study evaluated the influence of different storage duration )0, 1.5 and 5 h) at 5 ºC and type of cryoprotectant on freezability of ram epididymal spermatozoa. With increasing the s...
متن کاملMotility Parameters and Intracellular ATP Content of Rabbit Spermatozoa Stored for 3 Days at 15°C
GOGOL P. 2013. Motility parameters and intracellular ATP content of rabbit spermatozoa stored for 3 days at 15°C. Folia Biologica (Kraków) 61: 87-91. The effect of semen storage duration on motility parameters and ATP content of rabbit spermatozoa were investigated. Ejaculates were collected from 9 New Zealand White male rabbits and diluted with a commercial rabbit semen extender Galap. Semen w...
متن کاملFertility of ram spermatozoa pellet-frozen in zwitterion-buffered diluents.
The fertility (positive ultrasound scanning 60-75 days after insemination) of ram spermatozoa frozen in Tris- or zwitterion/glucose/egg yolk/glycerol-based diluents was examined during the natural breeding season (autumn). In Experiment 1 Tris-citrate, high glucose and Hepes-NaOH diluents were used and ewes (n = 266) were inseminated (induced oestrus) with 20 (uterus) or 180 (cervix) million mo...
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ژورنال
عنوان ژورنال: Animal
سال: 2015
ISSN: 1751-7311
DOI: 10.1017/s1751731114002109